An Amperometric Nitrite Biosensor Based on the Bioelectrocatalysis of Hemoglobin Incorporated in Sol-gel Film
نویسندگان
چکیده
A highly sensitive, fast and stable biosensor for determination of nitrite was developed using hemoglobin immobilized on a poly (N-isopropylacyamide-co-3methacryloxypropyltrimethoxysilane) (PNM) modified glass carbon electrodes. The matrix provided a biocompatible microenvironment for retaining the native structure and activity of the entrapped hemoglobin. Nitrite could be reduced by the electrocatalysis of the entrapped hemoglobin without any mediator. The reagentless sensor exhibited a fast response (less than 8 s) and sensitivity as high as 0.73 μA mM cm -2 . The linear range for nitrite determination was from 0.11 to 1.88 mM with a detection limit of 2.0×10 -5 mol L -1 .
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